Tidsskrift : Molecules , vol. 26 , 2021
Utgiver : MDPI
Trykt : 1420-3049
Elektronisk : 1420-3049
Publikasjonstype : Vitenskapelig artikkel
Sak : 17
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A study of the effects of single and combined protease hydrolysis on myofibrillar versus
collagenous proteins of poultry by-products has been conducted. The aim was to contribute with
knowledge for increased value creation of all constituents of these complex by-products. A rational
approach was implemented for selecting proteases exhibiting the most different activity towards the
major protein-rich constituents of mechanically deboned chicken residue (MDCR). An initial activity
screening of 18 proteases on chicken meat, turkey tendons and MDCR was conducted. Based on
weight yield, size exclusion chromatography (SEC) and SDS-PAGE, stem Bromelain and Endocut-02
were selected. Studies on hydrolysis of four different poultry by-products at 40 ◦C, evaluated by
protein yield, SEC, and SDS-PAGE, indicate that the proteases’ selectivity difference can be utilized
in tailor-making hydrolysates, enriched in either meat- and collagen-derived peptides or gelatin.
Three modes of stem Bromelain and Endocut-02 combinations during hydrolysis of MDCR were
performed and compared with single protease hydrolysis. All modes of the protease combinations
resulted in a similar approximately 15% increase in product yield, with products exhibiting similar
SEC and SDS-PAGE profiles. This shows that irrespective of the modes of combination, the use of
more than one enzyme in hydrolysis of collagen-rich material can provide means to increase the total
protein yield and ultimately contribute to increased value creation of poultry by-products.