Magnetic ligand fishing using immobilized DPP-IV for identification of antidiabetic ligands in lingonberry extract
Publikasjonsdetaljer
Tidsskrift : PLOS ONE , vol. 16 , p. 1–14 , 2021
Internasjonale standardnummer
:
Elektronisk
:
1932-6203
Publikasjonstype : Vitenskapelig artikkel
Sak : 2
Lenker
:
DOI
:
doi.org/10.1371/journal.pone.0...
ARKIV
:
hdl.handle.net/11250/2783611
Forskningsområder
Kvalitet og målemetoder
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Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no
Sammendrag
Article Authors Metrics Comments Media Coverage Abstract 1. Introduction 2. Materials and methods 3. Results and discussion 4. Conclusion Supporting information References Reader Comments (0) Figures Abstract In this work, a new magnetic ligand fishing probe for discovery of DPP-IV inhibitory ligands was developed and it was tested as a proof of concept on the fruit extract of Vaccinium vitis-idaea (lingonberry). The ligands were shown to have appreciable dipeptidyl peptidase IV (DPP-IV) inhibitory activity (IC50: 31.8 μg mL-1).) Inhibition of DPP-IV is a well-known therapeutic approach for management of type 2 diabetes (T2D). DPP-IV was successfully immobilized onto magnetic beads and was shown to retain its catalytic activity and selectivity over a model mixture. A total of four ligands were successfully fished out and identified as cyanidin-3-galactoside (2), cyanidin-3-arabinoside (3), proanthocynidin A (4), and 10-carboxyl-pyranopeonidin 3-O-(6″-O-p-coumaroyl)-glucoside (5) using HPLC/HRMS.