A fish intestinal in vitro model for investigation of lipid metabolism and steatosis
Publikasjonsdetaljer
Tidsskrift : Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids , vol. 1870 , p. 1–11 , 2025
Utgiver : Elsevier
Internasjonale standardnummer
:
Trykt
:
1388-1981
Elektronisk
:
1879-2618
Publikasjonstype : Vitenskapelig artikkel
Sak : 1
Lenker
:
DOI
:
doi.org/10.1016/j.bbalip.2024....
Forskningsområder
Fôrutvikling og ernæring
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Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no
Sammendrag
Choline is now recognized as an essential nutrient to ensure lipid transport in Atlantic salmon. Its deficiency leads to excessive lipid accumulation in the enterocytes, a condition known as steatosis. The knowledge of lipid metabolism and steatosis in fish remains limited, motivating the use of in vitro intestinal models to perform deeper explorations. This study aimed to create an in vitro steatosis model using RTdi-MI, a new cell line derived from the distal intestine of rainbow trout. Cells were exposed to varying oleic acid (OA) concentrations over different time points (24 h, 72 h, and 168 h). Results indicated that the increasing OA concentration enhanced intracellular lipid droplet formation. Quantitative lipid analysis confirmed OA accumulation, which intensified with prolonged exposure and increased OA dose. Moreover, all cells, including controls, exhibited fatty acid metabolic activity. Such outcome was confirmed by light and fluorescence microscopy. Additionally, RTdi-MI cells expressed genes inv
