Vitenskapelig artikkel

Development of 25 gene-associated microsatellite markers of Atlantic cod (Gadus morhua L.)

Stenvik, Jørgen; Breiland, Mette Serine W; Fjalestad, Kjersti Turid; Damsgård, Børge; Delghandi, Madjid

Publikasjonsdetaljer

Tidsskrift: Molecular Ecology Notes, vol. 6, p. 1105–1107, 2006

Internasjonale standardnumre:
Trykt: 1471-8278
Elektronisk: 1471-8286

Open Access: none

Lenker:
DOI

Microsatellites were identified by screening 2294 GenBank entries available for Atlantic
cod (
Gadus morhua
L.), mainly representing expressed sequence tags and cDNA sequences.
Ninety-two novel microsatellite loci (tetra-, tri- and dinucleotides) were characterized on 96
individuals. This strategy yielded 25 gene-associated polymorphic microsatellite markers
(11 tri- and 14 dinucleotides) with two to 20 alleles and an average heterozygosity of 0.48 in
the population studied (range 0.02–0.89). One marker exhibited significant homozygote
excess, and one of the primer pairs amplified two linked markers. The gene identity was
determined at nine of the loci, confirming the associated microsatellites as type I markers.