Development of 25 gene-associated microsatellite markers of Atlantic cod (Gadus morhua L.)
Publikasjonsdetaljer
Tidsskrift : Molecular Ecology Notes , vol. 6 , p. 1105–1107 , 2006
Internasjonale standardnummer
:
Trykt
:
1471-8278
Elektronisk
:
1471-8286
Publikasjonstype : Vitenskapelig artikkel
Lenker
:
DOI
:
doi.org/10.1111/j.1471-8286.20...
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Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no
Sammendrag
Microsatellites were identified by screening 2294 GenBank entries available for Atlantic cod ( Gadus morhua L.), mainly representing expressed sequence tags and cDNA sequences. Ninety-two novel microsatellite loci (tetra-, tri- and dinucleotides) were characterized on 96 individuals. This strategy yielded 25 gene-associated polymorphic microsatellite markers (11 tri- and 14 dinucleotides) with two to 20 alleles and an average heterozygosity of 0.48 in the population studied (range 0.02–0.89). One marker exhibited significant homozygote excess, and one of the primer pairs amplified two linked markers. The gene identity was determined at nine of the loci, confirming the associated microsatellites as type I markers.
