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Publisert 2006

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Publikasjonsdetaljer

Tidsskrift : Molecular and Cellular Probes , vol. 20 , p. 269–279–11 , 2006

Internasjonale standardnummer :
Trykt : 0890-8508
Elektronisk : 1096-1194

Publikasjonstype : Vitenskapelig artikkel

Bidragsytere : Skånseng, Beate; Kaldhusdal, Magne; Rudi, Knut

Sak : 5

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Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no

Sammendrag

We compared the colonisation of the chicken gut by the two important pathogens Campylobacter jejuni (frequent food-borne pathogen) and alpha-toxin gene containing Clostridium perfringens (causative agent of necrotic enteritis in chickens) using a new high-throughput automated DNA purification method for microbial biodiversity analyses. The method gave high reproducibility (standard deviation of 1.1 C-T-values for a universal 16S rDNA real-time PCR), and inhibition was observed in only 0.9% of the individual DNA purifications (n = 753). We analysed 253 randomly collected chicken caecal samples (sampled in 2001 and 2003) from Norwegian chicken flocks by real-time quantitative PCR. Our results showed positive correlation (P = 0.009) in chicken caccal colonisation between C jejuni and Cl. perfringens. We also found that there was a significant underrepresentation (P = 0.008) of chickens containing high levels of Cl. petfringens and low levels of C jejuni. This indicates a possible interaction between these bacteria. Potential interaction between pathogens and other bacteria in the gut will certainly be important research fields in the future. As demonstrated here, the development of new tools for high-throughput analyses will be of key importance for these studies. (c) 2006 Elsevier Ltd. All rights reserved.