Publisert 2023

Les på engelsk

Publikasjonsdetaljer

Tidsskrift : Food Microbiology , vol. 113 , p. 1–12 , 2023

Internasjonale standardnummer :
Trykt : 0740-0020
Elektronisk : 1095-9998

Publikasjonstype : Vitenskapelig artikkel

Bidragsytere : Fernández, Estefanía Noriega; Sivertsvik, Morten

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Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no

Sammendrag

Plasma-Activated Water (PAW) was generated from tap water using a surface dielectric barrier discharge at different discharge power (26 and 36 W) and activation time (5 and 30 min). The inactivation of a three-strain Listeria monocytogenes cocktail in planktonic and biofilm state was evaluated. PAW generated at 36 W-30 min showed the lowest pH and the highest hydrogen peroxide, nitrates, nitrites contents and effectiveness against cells on planktonic state, resulting in 4.6 log reductions after a 15-min treatment. Although the antimicrobial activity in biofilms formed on stainless steel and on polystyrene was lower, increasing the exposure time to 30 min allowed an inactivation >4.5 log cycles. The mechanisms of action of PAW were investigated using chemical solutions that mimic its physico-chemical characteristics and also RNA-seq analysis. The main transcriptomic changes affected carbon metabolism, virulence and general stress response genes, with several overexpressed genes belonging to the cobalamin-dependent gene cluster.

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