A 95 Peptidstørrelsesfordeling – Validering ny kolonne
Publikasjonsdetaljer
Utgiver : Nofima AS
Internasjonale standardnummer
:
Trykt
:
978-82-8296-699-3
Publikasjonstype : Nofimas rapportserie
Serier : Nofima rapportserie 36/2021
År : 2021
Lenker
:
ARKIV
:
hdl.handle.net/11250/2826199
Forskningsområder
Kvalitet og målemetoder
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Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no
Sammendrag
In the analysis ‘A 95 Molweight Distribution of Peptides’ BioLab use the Size Exclusion Chromatography (SEC) column Superdex Peptide. After a request for a new column of the same type it was informed that the column was no longer produced. The supplier recommended a replacement column, Superdex Increase. After a validation where the two columns was compared, it was concluded that BioLab could use Superdex Increase as a replacement for Superdex Peptide. This lead to a slight increase in the uncertainty for the methode, and a restriction in the measuring area. From ‘<200 – 20 000<’ to ‘<200 – 15 000<’.