Vitenskapelig artikkel

INFOGEST static in vitro simulation of gastrointestinal food digestion

Brodkorb, André; Egger, Lotti; Alminger, Marie; Alvito, Paula; Assunção, Ricardo; Ballance, Simon; Bohn, Torsten; Bourlieu-Lacanal, Claire; Boutrou, Rachel; Carrière, Frédéric; Clemente, Alfonso; Corredig, Milena; Dupont, Didier; Dufour, Claire; Edwards, Catharina; Golding, Matt; Karakaya, Sibel; Kirkhus, Bente; Le Feunteun, Steven; Lesmes, Uri; Macierzanka, Adam; Mackie, Alan R.; Martins, Carla; Marze, Sébastien; McClements, David Julian; Ménard, Olivia; Minekus, Mans; Portmann, Reto; Santos, Cláudia N.; Souchon, Isabelle; Singh, R. Paul; Vegarud, Gerd Elisabeth; Wickham, Martin S. J.; Weitschies, Werner; Recio, Isidra

Publikasjonsdetaljer

Tidsskrift: Nature Protocols, vol. 14, p. 991–1014, 2019

Utgiver: Nature Publishing Group

Internasjonale standardnumre:
Trykt: 1754-2189
Elektronisk: 1750-2799

Open Access: green

Lenker:
ARKIV
DOI

Developing a mechanistic understanding of the impact of food structure and composition on human health has
increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of
different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of
results. The standardized protocol presented here is based on an international consensus developed by the COST
INFOGEST network. The method is designed to be used with standard laboratory equipment and requires limited
experience to encourage a wide range of researchers to adopt it. It is a static digestion method that uses constant ratios of
meal to digestive fluids and a constant pH for each step of digestion. This makes the method simple to use but not
suitable for simulating digestion kinetics. Using this method, food samples are subjected to sequential oral, gastric and
intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on
available physiological data. This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated
with the original method, such as the inclusion of the oral phase and the use of gastric lipase. The method can be used to
assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids,
fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix. The whole protocol can be
completed in ~7 d, including ~5 d required for the determination of enzyme activities.