Publisert 2004

Les på engelsk


Tidsskrift : Journal of Applied Microbiology , vol. 96 , p. 201–208 , 2004

Internasjonale standardnummer :
Trykt : 1364-5072
Elektronisk : 1365-2672

Publikasjonstype : Vitenskapelig artikkel

Bidragsytere : Langsrud, Solveig; Sundheim, G.; Holck, Askild Lorentz

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Kjetil Aune


Aims: Study the effects of adaptation and stress on the resistance to benzalkonium chloride (BC) and cross-resistance to antibiotics in Escherichia coli. Methods and Results: Precultivation of E. coli ATCC 11775 and E. coli DSM 682 in the presence of sub-inhibitory concentrations of BC or stress inducers (salicylate, chenodeoxycholate and methyl viologen) resulted in higher MIC of BC and chloramphenicol (CHL). Adaptation to growth in six-fold of the initial MIC of BC resulted in stable BC-resistance and enhanced tolerance to several antibiotics and ethidium bromide (EtBr). The MIC of CHL increased more than tenfold for both strains. Enhanced efflux of EtBr in adapted E. coli ATCC 11775 indicated that the observed resistance was due to efflux. Changes in outer membrane protein profiles were detected in the BC-adapted cells. There were no indications of lower membrane permeability to BC. Conclusions: Induction of stress-response or gradual adaptation to BC or CHL results in acquired cross-tolerance between quaternary ammonium compounds and antibiotics in E. coli. Enhanced efflux was one of the observed differences in adapted cells. Significance and impact of the study: Provided not taking due precautions, extensive use of disinfectants could lead to emergence of antibiotic resistant isolates.