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Publisert 2006

Les på engelsk

Publikasjonsdetaljer

Tidsskrift : Molecular Ecology Notes , vol. 6 , p. 272–275 , 2006

Internasjonale standardnummer :
Trykt : 1471-8278
Elektronisk : 1471-8286

Publikasjonstype : Vitenskapelig artikkel

Bidragsytere : Stenvik, Jørgen; Breiland, Mette Serine W; Damsgård, Børge; Delghandi, Madjid

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Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no

Sammendrag

The two main allelic variants of the Atlantic cod ( Gadus morhua L.) pantophysin I ( Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. We present a new method for Pan I genotyping using fluorescent allelespecific duplex polymerase chain reaction (PCR). This method is more rapid, reliable and cost-effective than the previously published method and it is not affected by DNA source and quality. This improvement is important for studies demanding high throughput and accuracy of Pan I genotyping

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