Genotyping of pantophysin I (Pan I) of Atlantic cod (Gadus morhua L.) by allele-specific PCR
Publikasjonsdetaljer
Tidsskrift : Molecular Ecology Notes , vol. 6 , p. 272–275 , 2006
Internasjonale standardnummer
:
Trykt
:
1471-8278
Elektronisk
:
1471-8286
Publikasjonstype : Vitenskapelig artikkel
Lenker
:
DOI
:
doi.org/10.1111/j.1471-8286.20...
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Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no
Sammendrag
The two main allelic variants of the Atlantic cod ( Gadus morhua L.) pantophysin I ( Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. We present a new method for Pan I genotyping using fluorescent allelespecific duplex polymerase chain reaction (PCR). This method is more rapid, reliable and cost-effective than the previously published method and it is not affected by DNA source and quality. This improvement is important for studies demanding high throughput and accuracy of Pan I genotyping
