Vitenskapelig artikkel

Genotyping of pantophysin I (Pan I) of Atlantic cod (Gadus morhua L.) by allele-specific PCR

Stenvik, Jørgen; Breiland, Mette Serine W; Damsgård, Børge; Delghandi, Madjid

Publikasjonsdetaljer

Tidsskrift: Molecular Ecology Notes, vol. 6, p. 272–275, 2006

Internasjonale standardnumre:
Trykt: 1471-8278
Elektronisk: 1471-8286

Open Access: none

Lenker:
DOI

The two main allelic variants of the Atlantic cod (
Gadus morhua
L.) pantophysin I (
Pan
I)
locus have different frequencies within different cod stocks. The
Dra
I polymorphism
which distinguishes the two alleles can thus be used for discrimination of coastal and offshore
cod populations. We present a new method for
Pan
I genotyping using fluorescent allelespecific
duplex polymerase chain reaction (PCR). This method is more rapid, reliable and
cost-effective than the previously published method and it is not affected by DNA source
and quality. This improvement is important for studies demanding high throughput and
accuracy of
Pan
I genotyping