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Different length (DL) qPCR for quantification of cell killing by UV-induced DNA damage

Publisert 2010

Les på engelsk

Publikasjonsdetaljer

Tidsskrift : International Journal of Environmental Research and Public Health (IJERPH) , vol. 7 , p. 3376–3381–6 , 2010

Utgiver : MDPI

Internasjonale standardnummer :
Trykt : 1661-7827
Elektronisk : 1660-4601

Publikasjonstype : Vitenskapelig artikkel

Bidragsytere : Rudi, Knut; Hagen, Irina; Johnsrud, Bente Carina; Skjefstad, Guro; Tryland, Ingun

Sak : 9

Lenker :
OMTALE : http://www.mdpi.com/1660-4601/...
DOI : doi.org/10.3390/ijerph7093376

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Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no

Sammendrag

We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evaluated on three strains of Escherichia coli exposed to varying levels of ultraviolet (UV) radiation. We show that DL qPCR sensitivity and reproducibility are within the range of practical application to detect the effect of UV cell killing.