Publisert 2009

Les på engelsk


Tidsskrift : Electrophoresis , vol. 30 , p. 1856–1862–7 , 2009

Internasjonale standardnummer :
Trykt : 0173-0835
Elektronisk : 1522-2683

Publikasjonstype : Vitenskapelig artikkel

Bidragsytere : Grove, Harald; Mosleth, Ellen F.; Hollung, Kristin; Martens, Harald

Sak : 11

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Kjetil Aune


Silver staining is a commonly used protein stain to visualise proteins separated by 2-DE. Despite this, the technique suffers from a limited dynamic range, making the simultaneous quantification of high- and low-abundant proteins difficult. In this paper we take advantage of the fact that silver staining is not an end-point stain by photographing the gels during development. This procedure provides information about the change in measured absorbance for each pixel in the protein spots on the gel. The maximum rate of change was found to be correlated with the amount of applied protein, providing a new way of estimating protein amount in 2-DE gels. We observed an improvement in the dynamic range of silver staining by up to two orders of magnitude.