Vitenskapelig artikkel

FT-IR microspectroscopy: a promising method for the rapid identification of Listeria species

Janbu, Astrid Oust; Møretrø, Trond; Bertrand, Dominique; Kohler, Achim


Tidsskrift: FEMS Microbiology Letters, vol. 278, p. 164–170–7, 2008

Utgiver: Elsevier

Utgave: 2

Internasjonale standardnumre:
Trykt: 0378-1097
Elektronisk: 1574-6968

Open Access: none


This work presents a pilot study to investigate the potential of fourier transform infrared (FT-IR) microspectroscopy for rapid identification of Listeria at the species level. Using this technique, FT-IR spectra were acquired from 30 strains from five Listeria species. The FT-IR spectra were analysed using stepwise canonical discriminant analysis and partial least-squares regression in a stepwise identification scheme. The results showed that 93% of all the samples were assigned to the correct species, and that 80% of the Listeria monocytogenes strains were correctly identified. In comparison, 100% of the samples, including the L. monocytogenes samples, were correctly identified using spectra acquired by FT-IR macrospectroscopy. The results show that FT-IR microspectroscopy has potential as a rapid screening method for Listeria, which is especially valuable for the food industry.