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Publisert 2003

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Publikasjonsdetaljer

Tidsskrift : Scientific World Journal , vol. 3 , p. 578–584–7 , 2003

Internasjonale standardnummer :
Trykt : 1537-744X
Elektronisk : 1537-744X

Publikasjonstype : Vitenskapelig artikkel

Bidragsytere : Rudi, Knut; Treimo, Janneke; Nissen, Hilde; Vegarud, Gerd

Har du spørsmål om noe vedrørende publikasjonen, kan du kontakte Nofimas bibliotekleder.

Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no

Sammendrag

Analyses of complex microbial communities are becoming increasingly important. Bottlenecks in these analyses, however, are the tools to actually describe the biodiversity. Novel protocols for DNA array-based analyses of microbial communities are presented. In these protocols, the specificity obtained by sequence-specific labeling of DNA probes is combined with the possibility of detecting several different probes simultaneously by DNA array hybridization. The gene encoding 16S ribosomal RNA was chosen as the target in these analyses. This gene contains both universally conserved regions and regions with relatively high variability. The universally conserved regions are used for PCR amplification primers, while the variable regions are used for the specific probes. Protocols are presented for DNA purification, probe construction, probe labeling, and DNA array hybridizations.