Characterization, Production and Purification of Carnocin H, a Bacteriocin Produced by Carnobacterium 377
Publikasjonsdetaljer
Tidsskrift : Current Microbiology , vol. 43 , p. 227–231–5 , 2001
Utgiver : Springer
Internasjonale standardnummer
:
Trykt
:
0343-8651
Elektronisk
:
1432-0991
Publikasjonstype : Vitenskapelig artikkel
Sak : 4
Lenker
:
DOI
:
doi.org/10.1007/s002840010292
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Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no
Sammendrag
Carnocin H, a bacteriocin produced by a Carnobacterium sp., inhibited lactic acid bacteria, clostridia, enterococci, and some Staphylococcus aureus strains. Some strains of Listeria and Pediococcus were also sensitive to carnocin H. The bacteriocin was produced during the late stationary growth phase. Carnocin H was purified by cation exchange chromatography and reverse phase chromatography. Amino acid sequence and composition indicate that carnocin H is a novel bacteriocin belonging to the class II bacteriocins. The bacteriocin consists of approximately 75 amino acid residues with a highly cationic N-terminal containing six succeeding lysines. Activity, as measured by agar diffusion zones, was reduced at increased pH values, levels of indicator bacteria, NaCl, agar, and soy oil.