Vitenskapelig artikkel

Negative-ion electrospray ionisation-mass spectrometry (ESI-MS) as a tool for analysing structural heterogeneity in kappa-carrageenan oligosaccharides

Ekeberg, Dag; Knutsen, Svein Halvor; Sletmoen, Marit

Publikasjonsdetaljer

Tidsskrift: Carbohydrate Research, vol. 334, p. 49–59–11, 2001

Utgiver: Elsevier

Internasjonale standardnumre:
Trykt: 0008-6215
Elektronisk: 1873-426X

Open Access: none

Lenker:
OMTALE
DOI

Oligosaccharides, enzymically produced from kappa-carrageenan, have been investigated by electrospray ionisation mass spectrometry (ESI-MS). The technique was used without prior derivatisation of the oligosaccharide originally obtained by size exclusion chromatography (SEC). The structure of the oligosaccharides was mainly 4-sulphated neocarrabiose (A-G4S) with an increasing length ranging from di- to dodecasaccharides. However, in the larger oligosaccharides structural motivs deviating from the perfect alternating A-G4S structure were detected, i.e. (A2S-G4S). Although resulting in reduced signal intensity, samples added NaCl also gave rise to reliable mass spectra. Desulphation was induced at elevated cone voltages and in acidic or alkaline salt solutions.