Vitenskapelig artikkel

Thermal shock induction of triploidy in Atlantic cod (Gadus morhua L.)

Peruzzi, Stefano; Kettunen, Anne Helena; Primicerio, Raul; Kauric, Goran


Tidsskrift: Aquaculture Research, vol. 38, p. 926–932, 2007

Internasjonale standardnumre:
Trykt: 1355-557X
Elektronisk: 1365-2109

Open Access: none


The effects of thermal treatments on induction of triploidy
in Atlantic cod have been investigated. Cold
shock [-1.7 +/-0.1 1C at 20min post fertilization
(PF) for 2 h] was based on a previously developed protocol,
and heat shocks, below the lethal threshold of
24C, were at 16,18 or 20C applied 20,30 or 40min
PF for 20min. Cold shock did not a¡ect larval survival
and was ine¡ective for producing triploids (range
0-4%). A heat shock of 20C at 20min PF generated
the highest percentages (range 66-100%) of triploid
larvae at hatching, with survival ranging from 10%
to 20%relative to the controls. Lower heat shock temperatures or delayed shocks increased survival but
decreased the number of triploids, providing no net
gain in triploid yield (range 1-9%). Heat shocks applied
later than 20min PF produced 2-4% tetraploid
larvae at hatching. Athermal shock of 20C initiated
at 20min PF and lasting 20min proved to be the most
generally efficient treatment for induction of triploidy
in Atlantic cod.