Development of an inducible gene expression system for Lactobacillus sakei
Tidsskrift : Letters in Applied Microbiology , vol. 31 , p. 115–120 , 2003
Trykt : 0266-8254
Elektronisk : 1472-765X
Publikasjonstype : Vitenskapelig artikkel
DOI : doi.org/10.1046/j.1472-765X.20...
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Aim: To develop an inducible gene expression system for Lactobacillus sakei, based on the regulatory system of sakacin A production. Methods and Results: A Lactobacillus/Escherichia coli shuttle vector; pKRV3, was constructed including the signal transducing system genes of the bacteriocin sakacin A. The gusA gene fused to PsapA promoter, cloned in this vector allowed for inducible beta-glucuronidase expression in L. sakei and L. plantarum following the addition of the sakacin A inducing peptide. PsapA appeared to be a strong and tightly controlled promoter when compared with known promoters. Conclusion: The pKRV3 system can be used as an inducible gene expression system in lactobacilli. Significance and Impact of the Study: A novel, inducible gene expression system has been developed for lactic acid bacteria relevant in food fermentations.