Vitenskapelig artikkel

FT-IR microspectroscopy for early identification of some clinically relevant pathogens

Sandt, Christophe; Madoulet, C.; Kohler, Achim; Allouch, P.; De Champs, C.; Manfait, Michel; Sockalingum, Ganesh D.

Publikasjonsdetaljer

Tidsskrift: Journal of Applied Microbiology, vol. 101, p. 785–797, 2006

Utgave: 4

Internasjonale standardnumre:
Trykt: 1364-5072
Elektronisk: 1365-2672

Open Access: none

Lenker:
DOI

Aims:
To investigate the potentials and limitations of Fourier transform-infrared (FT-IR) microspectroscopy as a tool to identify, at the level of microcolonies, pathogenic bacteria frequently isolated in the clinical environment.

Methods and Results:
A total of 1570 FT-IR spectra from 164 gram-positive and gram-negative bacteria isolated from patients were recorded from 6 to 10-h old microcolonies of 50–150 lm size. A classification of 100% was obtained for the most frequent gram-positive bacteria, such as Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, and Enterococcus faecium at the species level. An average accuracy of about 80% was reached with Gram negative bacteria from the Enterobacteriaceae and Pseudomonaceae families; Enterobacter aerogenes, Enterobacter cloacae, Klebsiella spp., and Citrobacter koseri; and Proteus mirabilis and Escherichia coli. Results were comparable with FT-IR measurements on dried suspensions from 18-h cultures.

Conclusions:
Early identification of young microcolonies is feasible with FT-IR microscopy with a very high accuracy for gram-positive bacteria. Some improvement in the transfer of microcolonies is necessary to increase the accuracy for gram-negative bacteria.

Significance and Impact of the Study:
Combination of FT-IR microscopy and multivariate data analysis could be a complementary, rapid, and reliable tool for screening and discriminating, at species and subspecies level, micro-organisms of clinical, food-borne, or environmental origins.