Identification and characterisation of nine new gene-associated microsatellite markers for Atlantic cod (Gadus morhua L.)
Publikasjonsdetaljer
Tidsskrift : Conservation Genetics , p. 747–749 , 2008
Utgiver : Springer Nature
Internasjonale standardnummer
:
Trykt
:
1566-0621
Elektronisk
:
1572-9737
Publikasjonstype : Vitenskapelig artikkel
Sak : 9
Lenker
:
DOI
:
doi.org/10.1007/s10592-007-938...
Har du spørsmål om noe vedrørende publikasjonen, kan du kontakte Nofimas bibliotekleder.
Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no
Sammendrag
Nine polymorphic microsatellite markers were identified by screening of 2464 ESTs derived from a cDNA library of Atlantic cod (Gadus morhua L.). About 35 novel microsatellite loci were selected and characterised in 96 individual cod. Nine markers were successfully amplified with number of alleles from 3 to 18 per locus and the average heterozygosity was 0.57 in the panel examined (range 0.29-0.86). All loci followed the Hardy-Weinberg expectation and no significant linkage disequilibrium was found in a test including all pairwise combinations. The gene identity was determined at four of the loci, confirming the associated microsatellites as Type I markers.
