Vitenskapelig artikkel

Identification and characterisation of nine new gene-associated microsatellite markers for Atlantic cod (Gadus morhua L.)

Delghandi, Madjid; Stenvik, Jørgen; Nilsen, Frank; Breiland, Mette Serine W; Fjalestad, Kjersti Turid; Damsgård, Børge

Publikasjonsdetaljer

Tidsskrift: Conservation Genetics, p. 747–749, 2008

Utgiver: Springer

Utgave: 9

Internasjonale standardnumre:
Trykt: 1566-0621
Elektronisk: 1572-9737

Open Access: none

Lenker:
DOI

Nine polymorphic microsatellite markers were identified by screening of 2464 ESTs derived from a cDNA library of Atlantic cod (Gadus morhua L.). About 35 novel microsatellite loci were selected and characterised in 96 individual cod. Nine markers were successfully amplified with number of alleles from 3 to 18 per locus and the average heterozygosity was 0.57 in the panel examined (range 0.29-0.86). All loci followed the Hardy-Weinberg expectation and no significant linkage disequilibrium was found in a test including all pairwise combinations. The gene identity was determined at four of the loci, confirming the associated microsatellites as Type I markers.