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Publisert 2013

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Publikasjonsdetaljer

Tidsskrift : Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology , vol. 166 , p. 65–72 , 2013

Utgiver : Elsevier

Internasjonale standardnummer :
Trykt : 1096-4959
Elektronisk : 1879-1107

Publikasjonstype : Vitenskapelig artikkel

Bidragsytere : Randall, Kyla M.; Drew, Murray D.; Øverland, Margareth; Østbye, Tone-Kari K.; Bjerke, Målfrid Tofteberg; Vogt, Kjell gjermund; Ruyter, Bente

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Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no

Sammendrag

The aim of this study was to investigate the effects of petroselinic acid, found in coriander oil, on the ability of rainbow trout hepatocytes to increase the production of eicosapentaenoic acid (20:5n-3; EPA) and docosahexaenoic acid (22:6n-3; DHA) from [1-(14)C] α-linolenic acid (18:3n-3; ALA) and to reduce the production of arachidonic acid (20:4n-6; ARA) from [1-(14)C] 18:2n-6. Addition of coriander oil increased the production of 22:6n-3, from [1-(14)C] 18:3n-3, at the 0.5 and 1.0% inclusion levels and reduced the conversion of [1-(14)C] 18:2n-6 to 20:4n-6. β-Oxidation was significantly increased at the 1.5% inclusion level for [1-(14)C] 18:2n-6, however β-oxidation for [1-(14)C] 18:3n-3 only showed an increasing trend. Acetate, a main breakdown product of fatty acids (FA) via peroxisomal β-oxidation, decreased three-fold for [1-(14)C] 18:2n-6 and nearly doubled for [1-(14)C] 18:3n-3 when coriander was added at a 1.5% inclusion level. Acyl coenzyme A oxidase (ACO) enzyme activity showed no significant differences between treatments. Relative gene expression of ∆6 desaturase decreased with addition of coriander oil compared to the control. The addition of petroselinic acid via coriander oil to vegetable oil (VO) based diets containing no fishmeal (FM) or fish oil (FO), significantly increased the production of anti-inflammatory precursor 22:6n-3 (P=0.011) and decreased pro-inflammatory precursor 20:4n-6 (P=0.023) in radiolabelled hepatocytes of rainbow trout.

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