Histamine in fish: Liquid chromatographic determination with post-column derivatization and fluorescence detection
Publikasjonsdetaljer
Utgiver : Nofima AS
Internasjonale standardnummer
:
Trykt
:
978-82-8296-282-7
Publikasjonstype : Nofimas rapportserie
Serier : Nofima rapportserie 15/2015
År : 2015
Lenker
:
ARKIV
:
http://hdl.handle.net/11250/28...
FULLTEKST
:
nofimaas.sharepoint.com/sites/...
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Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no
Sammendrag
Histamine is formed by microbial decarboxylation of histidine. Histidine is an essential amino acid which is present in all fish and especially in fish tissues of Scomberiscida and Scombridae families, for example mackerel, herring, anchovy and tuna. Histamine may lead to Scombroid food poisoning, which resembles allergic reactions. This method is intended for quantification of histamine down to 2 mg/kg, which was determined to be a reasonable quantification limit. The method uses liquid chromatography with OPA (o-Phthaldialdehyde) as derivatization reagent followed by fluorescence detection. Recovery experiments showed that the recovery of histamine is good, between 97.7 and 102 %, for all tested sample matrixes and concentration levels (approximately 2-180 mg/kg). The method is fit for purpose.
