Tidsskriftspublikasjon » Vitenskapelig artikkel
Fourier transform infrared and Raman spectroscopy for characterization of Listeria monocytogenes strains
Applied and Environmental Microbiology ; Volume 72. p. 228–232. 2006
Oust, Astrid; Møretrø, Trond; Naterstad, Kristine; Sockalingum, Ganesh D.; Adt, Isabelle; Manfait, Michel; Kohler, Achim
The purpose of this study was to characterize the variation in biochemical composition of 89 strains of Listeria monocytogenes with different susceptibilities towards sakacin P, using Fourier transform infrared (FTIR) spectroscopy and Raman spectroscopy. The strains were also analyzed using amplified fragment length polymorphism (AFLP) analysis. Based on their susceptibilities to sakacin P, the 89 strains have previously been divided into two groups. Using the FTIR spectra and AFLP data, the strains were basically differentiated into the same two groups. Analyses of the FTIR and Raman spectra revealed that the strains in the two groups contained differences in the compositions of carbohydrates and fatty acids. The relevance of the variation in the composition of carbohydrates with respect to the variation in the susceptibility towards sakacin P for the L. monocytogenes strains is discussed.