Construction of vectors for inducible gene expression in Lactobacillus sakei and L. plantarum
Publikasjonsdetaljer
Tidsskrift : FEMS Microbiology Letters , vol. 229 , p. 119–126–8 , 2003
Utgiver : Oxford University Press
Internasjonale standardnummer
:
Trykt
:
0378-1097
Elektronisk
:
1574-6968
Publikasjonstype : Vitenskapelig artikkel
Lenker
:
DOI
:
doi.org/10.1016/S0378-1097(03)...
Har du spørsmål om noe vedrørende publikasjonen, kan du kontakte Nofimas bibliotekleder.
Kjetil Aune
Bibliotekleder
kjetil.aune@nofima.no
Sammendrag
We have constructed vectors for inducible expression of genes in Lactobacillus sakei and Lactobacillus plantarum. The key elements of these vectors are a regulatable promoter involved in the production of the bacteriocins sakacin A and sakacin P and the genes encoding the cognate histidine protein kinase and response regulator that are necessary to activate this promoter upon induction by a peptide pheromone. The vectors are built up of cassettes that permit easy exchange of all parts through restriction enzyme digestion and ligation. Using beta-glucuronidase as a reporter enzyme, variants of these vectors were compared with each other, and with a corresponding system based on genes involved in the production of nisin. Several of the new vectors permitted tightly controlled and efficient expression of beta-glucuronidase in both L. sakei and L. plantarum. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
