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Photoperiodic treatment promotes weight gain and influences gene expression of histone methyl transferases in Atlantic cod (Gadus morhua)

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Kjetil Aune

Bibliotekleder
kjetil.aune@nofima.no

Aquaculture Europe 2010; Porto, Portugal, 2010-10-05–2010-10-08

Nagasawa, Kazue; Giannetto, Alessia; Kirtiklis, Lech; Lazado, Carlo C.; Fernandes, Jorge

The photoperiodic treatment as typified by continuously illumination has become a standard operation in the aquaculture industry for a number of species, such as Atlantic salmon (Salmo salar) (Endal et al., 2000) or European sea bass (Dicentrachus labrax) (Begtashi et al., 2004). In Atlantic cod (Gadus morhua), it has been reported that continuous light treatment during the early juvenile stages has a large impact on harvesting size of Atlantic cod nearly 3 years later (Imsland et al., 2007). However, the molecular mechanisms regulating this process are still unclear. Therefore, we focused on the relationship between body growth and gene expression of histone methyltransferases (HMTs). Histone modifications may serve as an epigenetic regulation marking system that is responsible for maintaining the heritable programs of gene expression during ontogeny (Strahl and Allis, 2000). Particularly, histone methylation by mixed lineage leukemias (MLLs) plays critical role in gene expression and epigenetic regulation (Jenuwein and Allis, 2001). MLLs are HMTs that specifically methylate histone H3 at lysine 4 (H3K4) and linked gene activation (Hess, 2004). In mammals, there are seven MLL paralogues: MLL1, MLL2, MLL3, MLL4, MLL5, SETD1A, and SETD1B. In this study, we have cloned all mll orthologues in Atlantic cod. In addition, we had compared the mRNA expression levels of mll genes between two groups under different photoperiodic conditions.