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Genotyping of pantophysin I (Pan I) of Atlantic cod (Gadus morhua L.) by allele-specific PCR

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Kjetil Aune

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kjetil.aune@nofima.no

Molecular Ecology Notes ; Volume 6. p. 272–275. 2006

Stenvik, Jørgen; Breiland, Mette Serine Wesmajervi; Damsgård, Børge; Delghandi, Madjid

The two main allelic variants of the Atlantic cod ( Gadus morhua L.) pantophysin I ( Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. We present a new method for Pan I genotyping using fluorescent allelespecific duplex polymerase chain reaction (PCR). This method is more rapid, reliable and cost-effective than the previously published method and it is not affected by DNA source and quality. This improvement is important for studies demanding high throughput and accuracy of Pan I genotyping

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