Academic article

Evaluation of the Antibacterial Effect of a Triclosan-Containing Floor Used in the Food Industry

Møretrø, Trond; Sonerud, Tonje; Mangelrød, Ellen; Langsrud, Solveig

Publication details

Journal: Journal of Food Protection, vol. 69, p. 627–633, 2006

Issue: 3

International Standard Numbers:
Printed: 0362-028X
Electronic: 1944-9097

Open Access: none

Antibacterial surfaces are increasingly used in the food industry. In the present study, the antibacterial effect of a triclosancontaining
industrial floor was assessed. A poultry processing plant, which had a floor that contained triclosan, was visited,
and the floor was sampled for bacteria. A high bacterial diversity was found on the floor. Testing showed that bacteria isolated
from the floor showed a sensitivity to triclosan that covered a range of MICs from 0.07 to .40 ppm. Staphylococci were the
most sensitive, and Pseudomonas fluorescens and Serratia marcescens were the most tolerant. The MICs of triclosan for the
strains isolated from the floor were similar to the control strains from the corresponding genera or species of other origin.
Thus, the floor seemed not to select for strains that were tolerant to triclosan or that led to the development of resistance to
triclosan. Laboratory studies showed that the ability of bacteria to survive under dry conditions on coupons of the floor was
similar to that for stainless steel and that the survival of the bacteria on the floor was not linked to their tolerance of triclosan,
as determined by the MICs of triclosan. Adherence studies showed that bacteria were able to adhere to coupons of the floor;
however, no thick biofilm developed after 3 days of incubation. In an agar plate assay, the floor produced inhibition zones
against staphylococci, which are known to be very sensitive to triclosan, whereas no inhibition zones were observed for other
bacteria tested. In conclusion, the antibacterial effect of the floor seemed to be very low. Because the concentration of triclosan
in the floor was low compared to what has been reported for other triclosan-incorporated surfaces, sufficient amounts of
triclosan may not have been available on the surface of the floor to kill the bacteria.