Academic article

Effect of the fat composition of a single high-fat meal on inflammatory markers in healthy young women

Myhrstad, Mari; Narverud, Ingunn; Telle-Hansen, Vibeke Helen; Karhu, Toni; Bødtker-Lund, Daniel; Herzig, Karl-Heinz; Makinen, Markus; Halvorsen, Bente ; Retterstøl, Kjetil; Kirkhus, Bente; Granlund, Linda; Holven, Kirsten Bjørklund; Ulven, Stine Marie

Publication details

Journal: British Journal of Nutrition, vol. 106, p. 1826–1835–10, 2011

Publisher: Cambridge University Press

Issue: 12

International Standard Numbers:
Printed: 0007-1145
Electronic: 1475-2662

Open Access: none


The aim of the present study was to examine the effect of a single high-fat meal with different fat quality on circulating inflammatory markers and gene expression in peripheral blood mononuclear cells (PBMC) to elucidate the role of fat quality on postprandial inflammation.
A postprandial study with fourteen healthy females consuming three test meals with different fat quality was performed. Test days were
separated by 2 weeks. Fasting and postprandial blood samples at 3 and 6 h after intake were analysed. The test meal consisted of three
cakes enriched with coconut fat (43 % energy as saturated fat and 1 % energy as a-linolenic acid (ALA)), linseed oil (14 % energy as
ALA and 30 % energy as saturated fat) and cod liver oil (5 % energy as EPA and DHA and 5 % energy as ALA in addition to 31 % energy
as saturated fat). In addition, ex vivo PBMC experiments were performed in eight healthy subjects investigating the effects of EPA and
ALA on release and gene expression of inflammatory markers. The IL-8 mRNA level was significantly increased after intake of the cod
liver oil cake at 6 h compared with fasting level, which was significantly different from the effect observed after the intake of linseed
cake. In contrast, no effect was seen on circulating level of IL-8. In addition, ALA and EPA were shown to elicit different effects on
the release and mRNA expression levels of inflammatory markers in PBMC cultured ex vivo, with EPA having the most prominent proinflammatory potential