Academic article

Proteome Changes in Wheat Subjected to Different Nitrogen and Sulfur Fertilizations

Grove, Harald; Hollung, Kristin; Moldestad, Anette Aamodt; Mosleth, Ellen F.; Uhlen, Anne Kjersti

Publication details

Journal: Journal of Agricultural and Food Chemistry, vol. 57, p. 4250–4258–9, 2009

Publisher: American Chemical Society (ACS)

Issue: 10

International Standard Numbers:
Printed: 0021-8561
Electronic: 1520-5118

Open Access: none

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DOI

Controlling the quality of wheat for breadmaking is a major concern for the milling and baking industry. Wheat flour quality depends on both the genetic background and environmental factors during growth and storage. Amount and timing of application of fertilizer are factors that affect wheat quality. This study investigated the effect of different levels of nitrogen and sulfur on the tris-soluble and glutenin protein fractions by 2D-electrophoresis. Multivariate analysis was performed to study changes in the proteome pattern. In the tris-soluble fraction 20 proteins were changed in abundance due to S fertilization, whereas 16 proteins were changed in the glutenin protein fraction. It was found that induced sulfur deficiency during growth resulted in the most pronounced effect on protein composition. Understanding which proteins are affected by varying levels of fertilizers may help tailor specific traits in various wheat varieties.