Academic article

Phytochemical content in broccoli flower buds during postharvest storage and light treatments

Hagen, Sidsel Fiskaa; Schmidt, Gesine; Borge, Grethe Iren Andersen; Bengtsson, Gunnar

Publication details

Journal: Acta Horticulturae, vol. 1106, p. 61–66, 2015

International Standard Numbers:
Printed: 0567-7572

Open Access: none

Links:
DOI

The study aims to explore the effects of postharvest storage and light treatments
on the phytochemical content in broccoli flower buds, and also to compare different
methods for estimation of phytochemicals in fruits and vegetables. Freshly harvested
broccoli heads (Brassica oleracea var. italica ‘Ironman’) were pre-stored for 4 days at
4°C in the dark before storage for 6 days at 4 or 10°C. Light treatments with visible
light or a combination of visible light and UV-B irradiation were conducted during
storage at 10°C and for 12 h day-1. The content of glucosinolates and flavonols in
broccoli flower buds were determined by HPLC, whereas qualitative changes in
phytochemical profile were studied using a non-targeted metabolomics approach
based on UHPLC-ESI-qToF-MS. In addition, flavonol content during storage was
monitored by repeated measurements using a non-destructive method based on
chlorophyll fluorescence. The results show that the phytochemical content in flower
buds of broccoli heads stored under different light and temperature conditions differs
markedly in both quantity and composition.