Academic article

Two dimensional LC-MS/MS in detection of peptides in hypothalamus of the rat subjected to hypoxic stress

Mihailova, Albena; Karaszewski, Bartosz; Mosleth, Ellen F.; Hauser, Roman; Nyka, Walenty M.; Lundanes, Elsa; Greibrokk, Tyge

Publication details

Journal: Journal of Separation Science, vol. 31, p. 468–479, 2008

Publisher: Wiley-VCH Verlagsgesellschaft

International Standard Numbers:
Printed: 1615-9306
Electronic: 1615-9314

Open Access: none

Links:
DOI

A capillary 2-D LC method coupled with IT MS has been used for separation and
identification of peptides in rat hypothalamus. Animals of two different age groups
(8 and 50 wk) were exposed to two different rates of CO2 in inhaled air to investigate
the influence of different hypoxia/hypercapnia levels and their stress-related factor
on the peptide excretion. Peptide compounds were fractionated (strong cation
exchange chromatography), trapped, and separated (RP chromatography), and MS/
MS mass spectra were used for identification. About 107 peptide compounds were
identified and 88 of them were semiquantified. Among the characterized peptides,
there were fragments from proteins such as proenkephalin A, proSAAS, prosomatostatin,
prooxytocin, vasopressin, etc. Explorative principal component analysis (PCA)
combined with hypothesis testing was applied to the obtained data to investigate
the impact of age and hypoxic stress factors on the peptide pattern. Twenty-six peptides
revealed significant differences in concentrations between the animal groups
influenced by age and influx rate.